PromethION

PromethION

A High-Throughput, On-Demand, Ultra Long-Read DNA/RNA Sequencing System

PromethION is a standalone benchtop system designed to run up to 48 flow cells at any time. The system allows on-demand sequencing. Users can start and stop running individual experiments as required, or deploy multiple flow cells onto single experiments for greater speed or throughput.

Features:

  • High-throughput, high-sample number benchtop system
  • Modular: Up to 48 flow cells, each with up to 3,000 nanopore channels (total up to 144,000)
  • Flow cells may be run individually or concurrently
  • Same workflow as MinION at larger scale
  • Sample loading compatible with multi-channel pipettes
  • Real-time data analysis in the device
  • 4 sample inputs per flow cell, allowing loading of up to 192 libraries across the whole device

Integrated compute allows real-time basecalling and onward analysis.

Long reads

  • Choose your read length, from kbs to 100s of kbs
  • Easier assembly, phasing
  • Covering repetitive regions

Real time

  • Immediate access to data
  • Rapid time to result – move on
  • Rapid insight into sample quality

Easy, rapid prep

  • 1D library prep: <10mins, 2 pots
  • Low cost of materials
  • De-skill prep

On demand

  • Use as many of the 48 flow cells as you like
  • Barcode for even more samples
  • Run many experiments on one device

Accessible

  • Easy install
  • No additional lab infrastructure requirements

Nanopore sequencing offers advantages in all areas of research:

  • Microbiology
  • Environmental research
  • Microbiome
  • Basic genome research
  • Human genetics
  • Cancer research
  • Clinical research
  • Plant research
  • Transcriptome analysis
  • Population genomics
  • Animal research

Whole genome sequencing

  • De novo assembly
  • Scaffolding and finishing
  • Variant analysis: structural variation
  • Variant analysis: SNVs, phasing
  • Resequencing
  • Targeted sequencing

  • Panels – amplicons, sequence capture, exome
  • Variant analysis: structural variation
  • Variant analysis: SNVs, phasing
  • 16S rRNA analysis
  • RNA sequencing

  • Splice variant analysis
  • Transcriptome / gene expression
  • Fusion transcript analysis
  • Metagenomics

  • Real-time, unbiased analysis of mixed samples
  • Epigenetics

  • Methylation
  • Histone modification
  • Non-coding RNA activity
    • Linux OS
    • GridION OS
    • (MinKNOW inside)

    PromethION: A High-Throughput, On-Demand, Ultra Long-Read DNA/RNA Sequencing System